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验证数据展示

  • WB analysis using 85163-5-RR

    WB analysis using 85163-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85163-5-RR (Cd44 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of RAW 264.7 using 85163-5-RR

    IF Staining of RAW 264.7 using 85163-5-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Cd44 antibody (85163-5-RR, Clone: 242666C11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD44 Recombinant antibody

CD44 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85163-5-RR

产品说明书

CloneNo. 242666C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

ECMR-III, ECMR III, CSPG8, CDw44, CD44 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inC2C12 cells, HeLa cells, RAW 264.7 cells, A549 cells
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85163-5-RR targets CD44 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD44 antigen
别名 ECMR-III, ECMR III, CSPG8, CDw44, CD44 antigen
计算分子量86 kDa
观测分子量85 kDa
GenBank蛋白编号NM_009851.2
基因名称 Cd44
Gene ID (NCBI) 12505
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDNP_033981
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

实验方案

Product Specific Protocols
WB protocol for CD44 antibody 85163-5-RRDownload protocol
IF protocol for CD44 antibody 85163-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols