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验证数据展示

  • WB analysis using 86300-3-RR

    WB analysis using 86300-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86300-3-RR (CD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 86300-3-RR

    IHC staining of mouse spleen using 86300-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 86300-3-RR (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 86300-3-RR

    IHC staining of mouse spleen using 86300-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 86300-3-RR (CD4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse thymus using 86300-3-RR

    IHC staining of mouse thymus using 86300-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 86300-3-RR (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 86300-3-RR

    IF Staining of mouse spleen using 86300-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD4 antibody (86300-3-RR, Clone: 250263G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse thymus using 86300-3-RR

    IF Staining of mouse thymus using 86300-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CD4 antibody (86300-3-RR, Clone: 250263G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse thymus using 86300-3-RR

    IF Staining of mouse thymus using 86300-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CD4 antibody (86300-3-RR, Clone: 250263G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD4 Recombinant monoclonal antibody

CD4 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 86300-3-RR

产品说明书

CloneNo. 250263G12

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, ELISA

别名

CD4 antigen, L3T4, Ly 4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse thymus tissue, mouse spleen tissue
Positive IHC detected inmouse spleen tissue, mouse thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse thymus tissue, mouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86300-3-RR targets CD4 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg3847

Product name: Recombinant Mouse CD4 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 27-394 aa of NM_013488

Sequence: KTLVLGKEGESAELPCESSQKKITVFTWKFSDQRKILGQHGKGVLIRGGSPSQFDRFDSKKGAWEKGSFPLIINKLKMEDSQTYICELENRKEEVELWVFKVTFSPGTSLLQGQSLTLTLDSNSKVSNPLTECKHKKGKVVSGSKVLSMSNLRVQDSDFWNCTVTLDQKKNWFGMTLSVLGFQSTAITAYKSEGESAEFSFPLNFAEENGWGELMWKAEKDSFFQPWISFSIKNKEVSVQKSTKDLKLQLKETLPLTLKIPQVSLQFAGSGNLTLTLDKGTLHQEVNLVVMKVAQLNNTLTCEVMGPTSPKMRLTLKQENQEARVSEEQKVVQVVAPETGLWQCLLSEGDKVKMDSRIQVLSRGVNQT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD4 antigen
别名 CD4 antigen, L3T4, Ly 4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3
计算分子量51 kDa
观测分子量55 kDa
GenBank蛋白编号NM_013488
基因名称 Cd4
Gene ID (NCBI) 12504
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP06332
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
WB protocol for CD4 antibody 86300-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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