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验证数据展示

  • WB analysis using 84139-4-RR (same clone as 84139-4-PBS)

    WB analysis using 84139-4-RR (same clone as 84139-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84139-4-RR (CD3EAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84139-4-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 84139-4-RR (same clone as 84139-4-PBS)

    IF Staining of A431 using 84139-4-RR (same clone as 84139-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CD3EAP antibody (84139-4-RR, Clone: 241379H5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84139-4-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 84139-4-RR (same clone as 84139-4-PBS)

    FC experiment of U2OS using 84139-4-RR (same clone as 84139-4-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug CD3EAP Recombinant antibody (84139-4-RR, Clone:241379H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84139-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01056-3

    Cytometric bead array standard curve of MP01056-3

    Cytometric bead array standard curve of MP01056-3, CD3EAP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84139-4-PBS. Detection antibody: 84139-2-PBS. Standard: Ag35074. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84139-4-RR

    Affinity and Kinetic Characterization of 84139-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84139-4-RR against Human CD3EAP were performed. The affinity constant is 0.339 nM.

CD3EAP Recombinant antibody, PBS Only (Capture)

CD3EAP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84139-4-PBS

产品说明书

CloneNo. 241379H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

241379H5, A34.5, Antisense to ERCC-1 protein, ASE1, ASE-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84139-4-PBS targets CD3EAP as part of a matched antibody pair:

MP01056-3: 84139-4-PBS capture and 84139-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 CD3EAP fusion protein Ag35074 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD3e molecule, epsilon associated protein
别名 241379H5, A34.5, Antisense to ERCC-1 protein, ASE1, ASE-1
计算分子量 510 aa, 55 kDa
观测分子量80-90 kDa
GenBank蛋白编号BC038992
基因名称 CD3EAP
Gene ID (NCBI) 10849
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15446
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 and as CD3ɛ-associated signal transducer (CAST), CD3EAP is a 55 kDa nucleolar autoantigen that has an apparent molecular mass of 90 kDa (PMID: 11199923). CD3EAP/hPAF49 contains a single tyrosine residue at position 82 (Tyr82), which is phosphorylated upon stimulation of the T-cell receptor. Western blot analysis detected CD3EAP at an apparent molecular mass of ~80-90 kDa.