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验证数据展示

  • WB analysis using 85898-4-RR (same clone as 85898-4-PBS)

    WB analysis using 85898-4-RR (same clone as 85898-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 85898-4-RR (same clone as 85898-4-PBS)

    IHC staining of rat liver using 85898-4-RR (same clone as 85898-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 85898-4-RR (same clone as 85898-4-PBS)

    IHC staining of rat liver using 85898-4-RR (same clone as 85898-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

  • IF Staining of rat liver using 85898-4-RR (same clone as 85898-4-PBS)

    IF Staining of rat liver using 85898-4-RR (same clone as 85898-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CD31 antibody (85898-4-RR, Clone: 250372F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

Rat CD31 Recombinant monoclonal antibody, PBS Only

CD31 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 85898-4-PBS

产品说明书

CloneNo. 250372F8

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

Pecam, Pecam1, PECAM-1, Platelet endothelial cell adhesion molecule

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85898-4-PBS targets CD31 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg1456

Product name: recombinant rat CD31/PECAM-1 protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 18-589 aa of NP_113779.1

Sequence: QENSFTINSIHMESRPSWEVSNGQKLTLQCLVDISTTSKSRPQHQVLFYKDDALVYNVSSSEHTESFVIPQSRVFHAGKYKCTVILNSKEKTTIEYQLTVNGVPMPEVTVDKKEVTEGGIVTVNCSMQEEKPPIYFKIEKVELGTKNVKLSREKTSNMNFVLIEFPIEEQDHLLVFRCQAGVLSGIKMQTSEFIRSEYVTVQEFFSTPKFQIQPPEMIIEGNQLHIKCSVQVAHLAQEFPEIIIQKDKAIVATSKQSKEAVYSVMALVEHSGHYTCKVESNRISKASSILVNITELFPRPKLELSSSRLDQGEMLDLSCSVSGAPVANFTIQKEETVLSQYQNFSKIAEERDSGLYSCTAGIGKVVKRSNLVPVQVCEMLSKPRIFHDAKFEIIKGQIIGISCQSVNGTAPITYRLLRAKSNFQTVQKNSNDPVTFTDKPTRDMEYQCIVDNCHSHPEVRSEILRVKVIAPVDEVTISILSGNDVQSGDEMVLRCSVKEGTGPVTFQFYKEKEGRPFHEETVNDTQVFWHHEQTSKEQEGQYYCTAFNRASIVTSLRSGPLTVRVFLAPWKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 platelet/endothelial cell adhesion molecule 1
别名 Pecam, Pecam1, PECAM-1, Platelet endothelial cell adhesion molecule
计算分子量76kDa
观测分子量100-130 kDa
GenBank蛋白编号NP_113779.1
基因名称 Pecam1
Gene ID (NCBI) 29583
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ3SWT0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

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