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验证数据展示

  • WB analysis of EL-4 using 84420-7-RR (same clone as 84420-7-PBS)

    WB analysis of EL-4 using 84420-7-RR (same clone as 84420-7-PBS)

    EL-4 cells were subjected to SDS PAGE followed by western blot with 84420-7-RR (CD30/TNFRSF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84420-7-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84420-7-RR (same clone as 84420-7-PBS)

    IHC staining of mouse spleen using 84420-7-RR (same clone as 84420-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84420-7-RR (CD30/TNFRSF8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84420-7-PBS in a different storage buffer formulation.

Mouse CD30/TNFRSF8 Recombinant monoclonal antibody, PBS Only

CD30/TNFRSF8 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84420-7-PBS

产品说明书

CloneNo. 241710B9

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, Indirect ELISA

别名

CD30, CD30L receptor, Lymphocyte activation antigen CD30, Tnfrsf8, Tumor necrosis factor receptor superfamily member 8

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84420-7-PBS targets CD30/TNFRSF8 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1307

Product name: Recombinant Mouse CD30/TNFRSF8 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 19-281 aa of NM_009401.2

Sequence: FPTDRPLKTTCAGDLSHYPGEAARNCCYQCPSGLSPTQPCPRGPAHCRKQCAPDYYVNEDGKCTACVTCLPGLVEKAPCSGNSPRICECQPGMHCCTPAVNSCARCKLHCSGEEVVKSPGTAKKDTICELPSSGSGPNCSNPGDRKTLTSHATPQAMPTLESPANDSARSLLPMRVTNLVQEDATELVKVPESSSSKAREPSPDPGNAEKNMTLELPSPGTLPDISTSENSKEPASTASTLSLVVDAWTSSRMQPTSPLSTGT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 8
别名 CD30, CD30L receptor, Lymphocyte activation antigen CD30, Tnfrsf8, Tumor necrosis factor receptor superfamily member 8
计算分子量53kDa
观测分子量 105-120 kDa
GenBank蛋白编号NM_009401.2
基因名称 Tnfrsf8
Gene ID (NCBI) 21941
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ60846
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).

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