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验证数据展示

  • WB analysis of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

    WB analysis of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP01134-2

    Sandwich ELISA sample test of MP01134-2

    Serum of six mouse was measured. The CD23 concentration of detected samples was determined to be 2624.00 pg/mL with a range of 1279.00 - 7445.00 pg/mL.

  • Sandwich ELISA standard curve of MP01134-2

    Sandwich ELISA standard curve of MP01134-2

    Sandwich ELISA standard curve of MP01134-2, Mouse CD23 Recombinant Matched Antibody Pair - PBS only. 84212-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1295. 84212-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • IHC staining of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

    IHC staining of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

    IHC staining of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

    IF Staining of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD23 antibody (84212-3-RR, Clone: 241385C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

    IF Staining of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD23 antibody (84212-3-RR, Clone: 241385C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

  • IF Staining of mouse thymus using 84212-3-RR (same clone as 84212-3-PBS)

    IF Staining of mouse thymus using 84212-3-RR (same clone as 84212-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CD23 antibody (84212-3-RR, Clone: 241385C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

  • IF Staining of mouse thymus using 84212-3-RR (same clone as 84212-3-PBS)

    IF Staining of mouse thymus using 84212-3-RR (same clone as 84212-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CD23 antibody (84212-3-RR, Clone: 241385C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84212-3-RR

    Affinity and Kinetic Characterization of 84212-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84212-3-RR against Mouse CD23/Fc epsilon RII were performed. The affinity constant is 0.168 nM.

Mouse CD23 Recombinant monoclonal antibody, PBS Only (Detector)

CD23 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA
Cat No. 84212-3-PBS

产品说明书

CloneNo. 241385C9

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA

别名

241385C9, Fc-epsilon-RII, Fcer2, Fcer2a, Low affinity immunoglobulin epsilon Fc receptor

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84212-3-PBS targets CD23 as part of a matched antibody pair:

MP01134-2: 84212-4-PBS capture and 84212-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1295

Product name: Recombinant Mouse CD23 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*His

Domain: 51-331 aa of EDL21948.1

Sequence: TEKNLKQLGDTAIQNVSHVTKDLQKFQSNQLAQKSQVVQMSQNLQELQAEQKQMKAQDSRLSQNLTGLQEDLRNAQSQNSKLSQNLNRLQDDLVNIKSLGLNEKRTASDSLEKLQEEVAKLWIEILISKGTACNICPKNWLHFQQKCYYFGKGSKQWIQARFACSDLQGRLVSIHSQKEQDFLMQHINKKDSWIGLQDLNMEGEFVWSDGSPVGYSNWNPGEPNNGGQGEDCVMMRGSGQWNDAFCRSYLDAWVCEQLATCEISAPLASVTPTRPTPKSEP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc receptor, IgE, low affinity II, alpha polypeptide
别名 241385C9, Fc-epsilon-RII, Fcer2, Fcer2a, Low affinity immunoglobulin epsilon Fc receptor
计算分子量38kDa
观测分子量46 kDa
GenBank蛋白编号EDL21948.1
基因名称 Fcer2a
Gene ID (NCBI) 14128
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP20693-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD23/Fc ε RII) is a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21, involved in B cell differentiation and IgE regulation. Two forms of Fc ε RII (Fc ε RIIa and Fc ε RIIb) were found to exist, which differ in structure only in the N-terminal cytoplasmic region, and Fc ε RIIa and Fc ε RIIb play roles in effector cells of B-cell and IgE-mediated immunity, respectively. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, after IgE binding and antigen cross-linking, intracellular killing of parasites is induced by activation of the L-arginine-nitric oxide pathway.

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