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验证数据展示

  • WB analysis of human peripheral blood platelets using 82991-3-RR

    WB analysis of human peripheral blood platelets using 82991-3-RR

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 82991-3-RR (CD226 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 82991-3-RR

    IHC staining of human tonsillitis using 82991-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82991-3-RR (CD226 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEL using 82991-3-RR

    IF Staining of HEL using 82991-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HEL cells using CD226 antibody (82991-3-RR, Clone: 230041E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD226 Recombinant monoclonal antibody

CD226 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82991-3-RR

产品说明书

CloneNo. 230041E12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格价格库存

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经过测试的应用

Positive WB detected inhuman peripheral blood platelets
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEL cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82991-3-RR targets CD226 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD226 molecule
别名 DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen
计算分子量 336 aa, 39 kDa
观测分子量65 kDa
GenBank蛋白编号BC074787
基因名称 CD226
Gene ID (NCBI) 10666
ENSEMBL Gene IDENSG00000150637
RRIDAB_3670738
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ15762
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).

实验方案

Product Specific Protocols
IF protocol for CD226 antibody 82991-3-RRDownload protocol
IHC protocol for CD226 antibody 82991-3-RRDownload protocol
WB protocol for CD226 antibody 82991-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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