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Host
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Species Reactivity
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Compatible Species
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验证数据展示

  • WB analysis using 83254-1-RR (same clone as 83254-1-PBS)

    WB analysis using 83254-1-RR (same clone as 83254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83254-1-RR (CD19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 83254-1-RR (same clone as 83254-1-PBS)

    IHC staining of rat spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    IF Staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD19 antibody (83254-1-RR, Clone: 240135A3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83254-1-RR

    Affinity and Kinetic Characterization of 83254-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83254-1-RR against Mouse CD19 were performed. The affinity constant is 0.303 nM.

    {{ptg:ProductFeaturesInfo}}

Mouse CD19 Recombinant monoclonal antibody, PBS Only

CD19 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 83254-1-PBS

产品说明书

CloneNo. 240135A3

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

240135A3, B-lymphocyte antigen CD19, CD19 antigen

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83254-1-PBS targets CD19 in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg0907

Product name: Recombinant Mouse CD19 protein (His Tag)

Source: mammalian cells-derived, pCMV (S18)

Tag: C-6*HIS

Domain: 19-287 aa of NM_009844.2

Sequence: RPQKSLLVEVEEGGNVVLPCLPDSSPVSSEKLAWYRGNQSTPFLELSPGSPGLGLHVGSLGILLVIVNVSDHMGGFYLCQKRPPFKDIWQPAWTVNVEDSGEMFRWNASDVRDLDCDLRNRSSGSHRSTSGSQLYVWAKDHPKVWGTKPVCAPRGSSLNQSLINQDLTVAPGSTLWLSCGVPPVPVAKGSISWTHVHPRRPNVSLLSLSLGGEHPVREMWVWGSLLLLPQATALDEGTYYCLRGNLTIERHVKVIARSAVWLWLLRTGG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD19 antigen
别名 240135A3, B-lymphocyte antigen CD19, CD19 antigen
计算分子量60 kDa
观测分子量62-70 kDa
GenBank蛋白编号NM_009844.2
基因名称 Cd19
Gene ID (NCBI) 12478
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP25918
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD19 is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).