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Host
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验证数据展示

  • WB analysis using 84417-5-RR (same clone as 84417-5-PBS)

    WB analysis using 84417-5-RR (same clone as 84417-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84417-5-RR (CD157 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84417-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84417-5-RR (same clone as 84417-5-PBS)

    IHC staining of mouse spleen using 84417-5-RR (same clone as 84417-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84417-5-RR (CD157 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84417-5-PBS in a different storage buffer formulation.

  • IF Staining of RAW 264.7 using 84417-5-RR (same clone as 84417-5-PBS)

    IF Staining of RAW 264.7 using 84417-5-RR (same clone as 84417-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD157 antibody (84417-5-RR, Clone: 241822F10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84417-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84417-5-RR

    Affinity and Kinetic Characterization of 84417-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84417-5-RR against Mouse CD157 were performed. The affinity constant is 5.36 nM.

Mouse CD157 Recombinant antibody, PBS Only

CD157 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84417-5-PBS

产品说明书

CloneNo. 241822F10

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Bp3, Bone marrow stromal antigen 1, Antigen BP3, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84417-5-PBS targets CD157 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 bone marrow stromal cell antigen 1
别名 Bp3, Bone marrow stromal antigen 1, Antigen BP3, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2
计算分子量35kDa
观测分子量40-46 kDa
基因名称 Bst1
Gene ID (NCBI) 12182
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ64277
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD157 is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores. CD157 has a calculated molecular mass of 30-36 kDa and an apparent molecular mass of 40-46 kDa due to glycosylation (PMID: 8941363, 10491089).