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验证数据展示

  • WB analysis using 84247-4-RR

    WB analysis using 84247-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84247-4-RR (OX40/TNFRSF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84247-4-RR

    Affinity and Kinetic Characterization of 84247-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84247-4-RR against Human CD134/OX40 were performed. The affinity constant is 5.21 nM.

CD134/OX40 Recombinant antibody

CD134/OX40 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84247-4-RR

产品说明书

CloneNo. 241398B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

TNFRSF4, OX40L receptor, OX40, CD134, ACT35 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMJ cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84247-4-RR targets CD134/OX40 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 4
别名 TNFRSF4, OX40L receptor, OX40, CD134, ACT35 antigen
计算分子量 29 kDa
观测分子量45-50 kDa
GenBank蛋白编号NM_003327
基因名称 CD134
Gene ID (NCBI) 7293
RRIDAB_3671797
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP43489
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD134 (OX40) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule. CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmunity (PMID: 26215166). CD134 can interact with TRAF2, TRAF3, and TRAF5.

实验方案

Product Specific Protocols
WB protocol for CD134/OX40 antibody 84247-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols