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验证数据展示

  • WB analysis using 84474-5-RR

    WB analysis using 84474-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84474-5-RR (CD107b / LAMP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 84474-5-RR

    IHC staining of mouse liver using 84474-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84474-5-RR (CD107b / LAMP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 84474-5-RR

    IHC staining of mouse liver using 84474-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84474-5-RR (CD107b / LAMP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 84474-5-RR

    IF Staining of NIH/3T3 using 84474-5-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CD107b / LAMP2 antibody (84474-5-RR, Clone: 241893A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CD107b / LAMP2 Recombinant antibody

CD107b / LAMP2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84474-5-RR

产品说明书

CloneNo. 241893A7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CD107b, Lamp2, Mac3, Lamp 2, 241893A7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, RAW 264.7 cells, mouse kidney tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84474-5-RR targets CD107b / LAMP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lysosomal-associated membrane protein 2
别名 CD107b, Lamp2, Mac3, Lamp 2, 241893A7
计算分子量46 kDa
观测分子量90-110 kDa
GenBank蛋白编号NM_001017959.2
基因名称 CD107b / LAMP2
Gene ID (NCBI) 16784
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP17047-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

实验方案

Product Specific Protocols
WB protocol for CD107b / LAMP2 antibody 84474-5-RRDownload protocol
IHC protocol for CD107b / LAMP2 antibody 84474-5-RRDownload protocol
IF protocol for CD107b / LAMP2 antibody 84474-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols