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验证数据展示

  • WB analysis using 84474-3-RR

    WB analysis using 84474-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84474-3-RR (CD107b / LAMP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 84474-3-RR

    IF Staining of NIH/3T3 using 84474-3-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CD107b / LAMP2 antibody (84474-3-RR, Clone: 241893G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of mouse liver using 84474-3-RR

    IHC staining of mouse liver using 84474-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84474-3-RR (CD107b / LAMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD107b / LAMP2 Recombinant antibody

CD107b / LAMP2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84474-3-RR

产品说明书

CloneNo. 241893G7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CD107b, Lamp2, Mac3, Lamp 2, 241893G7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, mouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84474-3-RR targets CD107b / LAMP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lysosomal-associated membrane protein 2
别名 CD107b, Lamp2, Mac3, Lamp 2, 241893G7
计算分子量46 kDa
观测分子量90 kDa
GenBank蛋白编号NM_001017959.2
基因名称 CD107b / LAMP2
Gene ID (NCBI) 16784
RRIDAB_3671996
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP17047-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.

实验方案

Product Specific Protocols
WB protocol for CD107b / LAMP2 antibody 84474-3-RRDownload protocol
IHC protocol for CD107b / LAMP2 antibody 84474-3-RRDownload protocol
IF protocol for CD107b / LAMP2 antibody 84474-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols