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验证数据展示

  • IHC staining of human colon cancer using 32374-1-AP

    IHC staining of human colon cancer using 32374-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 32374-1-AP (CARD10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CARD10 Polyclonal antibody

CARD10 Polyclonal Antibody for IHC, ELISA
Cat No. 32374-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

BIMP1, CARD-containing MAGUK protein 3, Carma 3, CARMA3, Caspase recruitment domain-containing protein 10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32374-1-AP targets CARD10 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 CARD10 fusion protein Ag36178 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caspase recruitment domain family, member 10
别名 BIMP1, CARD-containing MAGUK protein 3, Carma 3, CARMA3, Caspase recruitment domain-containing protein 10
计算分子量 1032 aa, 116 kDa
GenBank蛋白编号BC113659
基因名称 CARD10
Gene ID (NCBI) 29775
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9BWT7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CARD10 antibody 32374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols