验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
26326-1-AP targets C17orf75 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag23638 Product name: Recombinant human C17orf75 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 32-396 aa of BC035715 Sequence: PPSSSHYCLYSYRGSRLAQQRGDSEDGSPSGTNAETPSGDDFSLSLADTNLPSEVEPELRSFIAKRLSRGAVFEGLGNVASVELKIPGYRVGCYYCLFQNEKLLPETVTIDSERNPSEYVVCFLGGSEKGLELFRLELDKYIQGLKNNMNCEARGLESHIKSYLSSWFEDVVCPIQRVVLLFQEKLTFLLHAALSYTPVEVKESDEKTKRDINRFLSVASLQGLIHEGTMTSLCMAMTEEQHKSVVIDCSSSQPQFCNAGSNRFCEDWMQAFLNGAKGGNPFLFRQVLENFKLKAIQDTNNLKRFIRQAEMNHYALFKCYMFLKNCGSGDILLKIVKVEHEEMPEAKNVIAVLEEFMKEALDQSF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 17 open reading frame 75 |
| 别名 | NJMU R1, Protein Njmu R1, Protein Njmu-R1 |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC035715 |
| 基因名称 | C17orf75 |
| Gene ID (NCBI) | 64149 |
| RRID | AB_3085857 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HAS0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C17orf75, also known as Njmu-R1, mainly localizes to cytoplasmic vesicles and the trans-Golgi network (TGN). This protein is a component of the WDR11 complex (the WDR11-FAM91A1-C17orf75 trimer) and cooperates with TBC1D23 to promote the Golgi capture and tethering of AP-1-derived vesicles. In cancer research, C17orf75 is significantly overexpressed in various malignant tumors, including hepatocellular carcinoma, and is associated with poor prognosis and advanced clinicopathological features. Knockdown of C17orf75 inhibits tumor cell proliferation, migration, and invasion, induces G2 phase arrest, and suppresses epithelial-mesenchymal transition (EMT). This protein is an emerging molecule in studies of vesicular transport, tumor progression, and reproductive biology.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for C17orf75 antibody 26326-1-AP | Download protocol |
| WB protocol for C17orf75 antibody 26326-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
