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验证数据展示

  • WB analysis of MCF-7 using 27431-1-AP

    WB analysis of MCF-7 using 27431-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27431-1-AP (BRAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 27431-1-AP

    IHC staining of human lung cancer using 27431-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 27431-1-AP

    IHC staining of human lung cancer using 27431-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of MCF-7 using 27431-1-AP

    IP experiment of MCF-7 using 27431-1-AP

    IP result of anti-BRAP (IP:27431-1-AP, 4ug; Detection:27431-1-AP 1:1000) with MCF-7 cells lysate 3200 ug.

  • IHC staining of mouse testis using 27431-1-AP

    IHC staining of mouse testis using 27431-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 27431-1-AP

    IHC staining of mouse testis using 27431-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BRAP Polyclonal antibody

BRAP Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 27431-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA

别名

BRAP, BRAP2, BRCA1 associated protein, IMP, RING finger protein 52, RNF52

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, A431 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27431-1-AP targets BRAP in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26728

Product name: Recombinant human BRAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 376-535 aa of BC136698

Sequence: RLVASKTDGKIVQYECEGDTCQEEKIDALQLEYSYLLTSQLESQRIYWENKIVRIEKDTAEEINNMKTKFKETIEKCDNLEHKLNDLLKEKQSVERKCTQLNTKVAKLTNELKEEQEMNKCLRANQVLLQNKLKEEERVLKETCDQKDLQITEIQEQLRD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BRCA1 associated protein
别名 BRAP, BRAP2, BRCA1 associated protein, IMP, RING finger protein 52, RNF52
计算分子量 592 aa, 67 kDa
观测分子量 62-70 kDa, 48 kDa
GenBank蛋白编号BC136698
基因名称 BRAP
Gene ID (NCBI) 8315
RRIDAB_2880869
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z569
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for BRAP antibody 27431-1-APDownload protocol
IP protocol for BRAP antibody 27431-1-APDownload protocol
WB protocol for BRAP antibody 27431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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