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验证数据展示

  • WB analysis using 82742-3-RR (same clone as 82742-3-PBS)

    WB analysis using 82742-3-RR (same clone as 82742-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82742-3-RR (BOP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82742-3-RR (same clone as 82742-3-PBS)

    WB analysis of HeLa using 82742-3-RR (same clone as 82742-3-PBS)

    WB result of BOP1 antibody (82742-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BOP1 transfected HeLa cells. This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 82742-3-RR (same clone as 82742-3-PBS)

    IHC staining of human ovarian cancer using 82742-3-RR (same clone as 82742-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82742-3-RR (BOP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BOP1 antibody included
  • IHC staining of human ovarian cancer using 82742-3-RR (same clone as 82742-3-PBS)

    IHC staining of human ovarian cancer using 82742-3-RR (same clone as 82742-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82742-3-RR (BOP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BOP1 antibody included
  • IF Staining of HepG2 using 82742-3-RR (same clone as 82742-3-PBS)

    IF Staining of HepG2 using 82742-3-RR (same clone as 82742-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BOP1 antibody (82742-3-RR, Clone: 3A4 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

BOP1 Recombinant monoclonal antibody, PBS Only

BOP1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82742-3-PBS

产品说明书

CloneNo. 3A4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KIAA0124, block of proliferation 1, 3A4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BOP1 Ready-To-Use IHC Kit. BOP1 primary antibody included.

产品信息

82742-3-PBS targets BOP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26669

Product name: Recombinant human BOP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-135 aa of BC013787

Sequence: MAGSRGAGRTAAPSVRPEKRRSEPELEPEPEPEPPLLCTSPLSHSTGSDSGVSDSEESVFSGLEDSGSDSSEDDDEGDEEGEDGALDDEGHSGIKKTTEEQVQASTPCPRTEMASARIGDEYAEDSSDEEDIRNT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 block of proliferation 1
别名 KIAA0124, block of proliferation 1, 3A4
计算分子量 84 kDa
观测分子量 117 kDa
GenBank蛋白编号BC013787
基因名称 BOP1
Gene ID (NCBI) 23246
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14137
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Block of Proliferation 1(BOP1) protein was first isolated from a mouse, and the truncation protein may led to cell cycle arrest. Bop1 plays a role in processing of the 28S and 5.8S rRNAs (ribosomal RNAs) to regulate ribosome biogenesis. And Bop1 also act as a component of Pes1/Bop1/WDR21 (PeBoW) complex for mature 60S ribosome, and consequently acts during cell division at G1 checkpoints (PMID:18670790; PMID:26940494). What's more, abnormal expression of BOP1 was associated with liver or colorectal cancers (PMID:16804918). The molecular weight of the protein we detected was slightly higher, about 117 kd, which is also seen in the article (PMID: 30100995).

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