BLNK Monoclonal antibody

BLNK Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66953-1-Ig

产品说明书

CloneNo. 1A1A1

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

B cell linker, B cell linker protein, BASH, BLNK, BLNK S, Cytoplasmic adapter protein, LY57, SLP 65, SLP65

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inhuman spleen tissue, Raji cells, Daudi cells, Ramos cells, pig spleen tissue
Positive IHC detected inhuman appendicitis tissue, mouse thymus tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy BLNK Ready-To-Use IHC Kit. BLNK primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:3000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66953-1-Ig targets BLNK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, pig, mouse, rat
免疫原 BLNK fusion protein Ag28673 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 B-cell linker
别名 B cell linker, B cell linker protein, BASH, BLNK, BLNK S, Cytoplasmic adapter protein, LY57, SLP 65, SLP65
计算分子量 50 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC018906
基因名称 BLNK
Gene ID (NCBI) 29760
RRIDAB_2882276
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8WV28
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BLNK (also known as SLP-65 or BASH) is an important adaptor protein selectively expressed in B-lineage cells. BLNK bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. BLNK has been shown to be necessary for BCR-mediated Ca2+ mobilization, for the activation of mitogen-activated protein kinases such as ERK, JNK, and p38 in a chicken B cell line DT40, and for activation of transcription factors such as NF-AT and NF-κB in human or mouse B cells. BLNK plays a crucial role in pre-BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.

实验方案

Product Specific Protocols
WB protocol for BLNK antibody 66953-1-IgDownload protocol
IHC protocol for BLNK antibody 66953-1-IgDownload protocol
IF protocol for BLNK antibody 66953-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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