验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, SW 1990 cells，Pig pancreas tissue, Rat pancreas tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68092-1-Ig targets MIST1 in WB, ELISA applications and shows reactivity with Human, Pig, Rat , Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Pig, Rat , Mouse |
| 免疫原 | MIST1 fusion protein Ag18509 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | basic helix-loop-helix family, member a15 |
| 别名 | BHLHA15, MIST 1, MIST1 |
| 计算分子量 | 189 aa, 21 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC113394 |
| 基因名称 | MIST1 |
| Gene ID (NCBI) | 168620 |
| RRID | AB_2918829 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q7RTS1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Muscle intestine stomach expression 1 (MIST1), also named bHLHa15, is a secretory cell transcription factor that belongs to the basic helix-loop-helix superfamily. MIST1 is mainly expressed in serous secreted cells and essential for the development and maturation of secretory cells. Secretory cells lacking MIST1 exhibit structural and functional defects, including alterations in cytoskeletal organization and cell polarity and improper cell-cell junction. In healthy pancreata, MIST1 facilitates cell-to-cell communication, promotes acinar cell polarity, controls Ca2+ flux, promotes unfolded protein response (UPR) homeostasis, aids in vesicle assembly, and is required for regulated exocytosis. MIST1 was downregulated in pancreatic cancer tissues compared with normal or adjacent pancreatic tissues and lower MIST1 expression in pancreatic cancer cell lines than normal acinar cells (BxPC-3, PANC-1, SW 1990) (PMID:29859299). MIST1 is downregulated in the development of gastric cancer and in gastric cancer cells compared with SW620 cells (PMID:34149921).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MIST1 antibody 68092-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |