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BAK Polyclonal antibody

BAK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 30017-1-AP

Print datasheet

Synonyms

Apoptosis regulator BAK, BAK, BAK LIKE, BAK1, CDN1

验证数据展示

  • WB analysis using 30017-1-AP

    WB analysis using 30017-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30017-1-AP (BAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30017-1-AP

    WB analysis using 30017-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30017-1-AP (BAK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, Neuro-2a cells, PC-12 cells, HeLa cells, THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy BAK Ready-To-Use IHC Kit. BAK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30017-1-AP targets BAK in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, ELISA
Tested Reactivity Human, rat, mouse
Immunogen BAK fusion protein Ag27958 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BCL2-antagonist/killer 1
Synonyms Apoptosis regulator BAK, BAK, BAK LIKE, BAK1, CDN1
Calculated Molecular Weight 23 kDa
Observed Molecular Weight23 kDa
GenBank Accession NumberBC004431
Gene Symbol BAK1
Gene ID (NCBI) 578
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16611
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

实验方案

Product Specific Protocols
WB protocol for BAK antibody 30017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols