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验证数据展示

  • WB analysis using 14200-1-AP

    WB analysis using 14200-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14200-1-AP (B3GNT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

B3GNT2 Polyclonal antibody

B3GNT2 Polyclonal Antibody for WB, ELISA
Cat No. 14200-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

b3Gal T7, B3GALT7, B3GN T1, B3GN T2, B3GNT, B3GNT 2, B3GNT1, B3GNT2, Beta 1,3 GalTase 7, Beta 1,3 Gn T1, Beta 1,3 Gn T2, Beta 3 Gx T7, Beta3Gal T7, Beta3GalT7, Beta3Gn T1, Beta3Gn T2, BETA3GNT, BGnT 1, BGnT 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, MCF-7 cells, SMMC-7721 cells, mouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14200-1-AP targets B3GNT2 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 B3GNT2 fusion protein Ag5404 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
别名 b3Gal T7, B3GALT7, B3GN T1, B3GN T2, B3GNT, B3GNT 2, B3GNT1, B3GNT2, Beta 1,3 GalTase 7, Beta 1,3 Gn T1, Beta 1,3 Gn T2, Beta 3 Gx T7, Beta3Gal T7, Beta3GalT7, Beta3Gn T1, Beta3Gn T2, BETA3GNT, BGnT 1, BGnT 2
计算分子量 9 kDa
观测分子量69 kDa
GenBank蛋白编号BC030579
基因名称 B3GNT2
Gene ID (NCBI) 10678
RRIDAB_3085431
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NY97
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-1,3-N-acetylglucosaminyltransferase (B3GNT2) is the most abundant and ubiquitously expressed B3GNT isoform and displays the greatest in vitro activity for N-glycan and O-glycan poly-LacNAc extensionis. B3GNT2 encodes a poly-N-acetyllactosamine synthase that targets >10 ligands and receptors to disrupt interactions between tumor and T cells and reduce T cell activation (PMID: 35338135, 32663509). B3GNT2 also has five predicted N-glycosylation sites at asparagine residues 79, 89, 127, 173, and 219 (PMID: 33229435). Western blots indicated the glycosylated form of B3GNT2 is detected at ~ 67 kDa.

实验方案

Product Specific Protocols
WB protocol for B3GNT2 antibody 14200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols