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验证数据展示

  • WB analysis using 83728-4-RR (same clone as 83728-4-PBS)

    WB analysis using 83728-4-RR (same clone as 83728-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83728-4-RR (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83728-4-RR (same clone as 83728-4-PBS)

    IHC staining of mouse brain using 83728-4-RR (same clone as 83728-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83728-4-RR (same clone as 83728-4-PBS)

    IHC staining of mouse brain using 83728-4-RR (same clone as 83728-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 83728-4-RR (same clone as 83728-4-PBS)

    IHC staining of mouse liver using 83728-4-RR (same clone as 83728-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 83728-4-RR (same clone as 83728-4-PBS)

    IHC staining of mouse liver using 83728-4-RR (same clone as 83728-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 83728-4-RR (same clone as 83728-4-PBS)

    IHC staining of rat cerebellum using 83728-4-RR (same clone as 83728-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83728-4-RR (same clone as 83728-4-PBS)

    IF Staining of HepG2 using 83728-4-RR (same clone as 83728-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apoe antibody (83728-4-RR, Clone: 240786C3 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83728-4-RR

    Affinity and Kinetic Characterization of 83728-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83728-4-RR against Mouse Apolipoprotein E were performed. The affinity constant is 0.793 nM.

Mouse Apoe Recombinant monoclonal antibody, PBS Only

Apoe Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83728-4-PBS

产品说明书

CloneNo. 240786C3

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

apolipoprotein E, AI255918, 240786C3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83728-4-PBS targets Apoe in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse, rat
免疫原

Fusion Protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Apolipoprotein E
别名 apolipoprotein E, AI255918, 240786C3
计算分子量36kDa
观测分子量36 kDa
GenBank蛋白编号NM_001305819.1
基因名称 Apoe
Gene ID (NCBI) 11816
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP08226
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apolipoprotein E (Apoe) is a core component of plasma lipoproteins that participates in diverse biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immunoregulation, and neuronal growth and repair (PMID: 17854398). Mouse apoE, like apoE4, contains the equivalent of Arg-112 and Glu-255, but lacks the critical Arg-61 equivalent (it contains Thr-61). Thus, mouse apoE does not display apoE4 domain interaction (PMID: 11553788).

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