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验证数据展示

  • WB analysis using 85955-1-RR

    WB analysis using 85955-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85955-1-RR (Annexin A13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85955-1-RR

    Affinity and Kinetic Characterization of 85955-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85955-1-RR against Human Annexin A13 were performed. The affinity constant is 6.29 nM.

    {{ptg:ProductFeaturesInfo}}

Annexin A13 Recombinant monoclonal antibody

Annexin A13 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 85955-1-RR

产品说明书

CloneNo. 250350A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

ANXA13, Annexin 13, Annexin XIII, Annexin-13, ANX13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse small intestine tissue, rat small intestine tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85955-1-RR targets Annexin A13 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18581

Product name: Recombinant human ANXA13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-316 aa of BC125158

Sequence: MGNRHAKASSPQGFDVDRDAKKLNKACKGMGTNEAAIIEILSGRTSDERQQIKQKYKATYGKELEEVLKSELSGNFEKTALALLDHPSEYAARQLQKAMKGLGTDESVLIEVLCTRTNKEIIAIKEAYQRLFDRSLESDVKGDTSGNLKKILVSLLQANRNEGDDVDKDLAGQDAKDLYDAGEGRWGTDELAFNEVLAKRSYKQLRATFQAYQILIGKDIEEAIEEETSGDLQKAYLTLVRCAQDCEDYFAERLYKSMKGAGTDEETLIRIIVTRAEVDLQGIKAKFQEKYQKSLSDMVRSDTSGDFRKLLVALLH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 annexin A13
别名 ANXA13, Annexin 13, Annexin XIII, Annexin-13, ANX13
计算分子量 316 aa, 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC125158
基因名称 Annexin A13
Gene ID (NCBI) 312
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP27216
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A13 (ANXA13) is believed to be the original founder gene of the 12-member vertebrate annexin A family, and it has acquired an intestine-specific expression associated with a highly differentiated intracellular transport function (PMID: 11961095). ANXA13, which is highly expressed in the digestive system and believed to be the original founder gene of the annexin A family (PMID: 28423508). Limited evidence suggests that ANXA13 may play a role in apoptotic cell-mediated immunosuppression, hypertension, and cell death recognition (PMID: 28423508). ANXA13 is highly expressed in the human colon adenocarcinoma cell line HT29.

实验方案

Product Specific Protocols
WB protocol for Annexin A13 antibody 85955-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols