Actin-Chromobody® plasmid (TagRFP)

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagRFP.

Catalog No:
acr

Applications:
Live cell imaging

Specificity:
Actin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii

Vector type:
Mammalian expression vector

Promoter:
Constitutive CMV IE

Reporter:
TagRFP

Codon usage:
Mammalian

Selection:
Kan/Neo

Encoded Nanobody/ VHH:
Monoclonal anti-Actin single domain antibody (sdAb) fragment

Sequence:
With the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to nuclear Actin fused to TagRFP or TagGFP2, as well as the full vector sequence.

Microscopy techniques:
Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED

Transfection:
Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.

Storage:
Shipped at ambient temperature. Store at -20 °C

Synonyms:
Actin Chromobody, Chromobody

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