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ASC/TMS1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 12.5-800 ng/mL

Cat no : MP03567-1

Synonyms

Pycard, Apoptosis-associated speck-like protein containing a CARD, Asc, CARD5, Caspase recruitment domain-containing protein 5

Validation Data Gallery

  • Sandwich ELISA standard curve of MP03567-1, Human ASC/TMS1 Recombinant Matched Antibody Pair - PBS only. 88328-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0774. 88328-2-PBS was HRP conjugated as the detection antibody. Range: 12.5-800 ng/mL

    Sandwich ELISA standard curve of MP03567-1

    Sandwich ELISA standard curve of MP03567-1, Human ASC/TMS1 Recombinant Matched Antibody Pair - PBS only. 88328-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0774. 88328-2-PBS was HRP conjugated as the detection antibody. Range: 12.5-800 ng/mL

  • The mean ASC/TMS1 concentration was determined to be 515.36 ng/mL in THP-1 cell extract based on a 1.5 mg/mL extract load, 424.39 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 821.97 ng/mL in HL-60 cell extract based on a 1.2 mg/mL extract load and 140.95 ng/mL in SK-BR-3 cell extract based on a 1.5 mg/mL extract load.

    Sandwich ELISA sample test of MP03567-1

    The mean ASC/TMS1 concentration was determined to be 515.36 ng/mL in THP-1 cell extract based on a 1.5 mg/mL extract load, 424.39 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 821.97 ng/mL in HL-60 cell extract based on a 1.2 mg/mL extract load and 140.95 ng/mL in SK-BR-3 cell extract based on a 1.5 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP03567-1 targets ASC/TMS1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 12.5-800 ng/mL (Sandwich ELISA);

Capture antibody: TMS1 Recombinant monoclonal antibody, PBS Only (Capture) 88328-3-PBS (260842D11). 100 μg. Concentration 1 mg/ml.

Detection antibody: TMS1 Recombinant monoclonal antibody, PBS Only (Detector) 88328-2-PBS (260842D12). 100 μg. Concentration 1 mg/ml.

Alternative ASC/TMS1 matched antibody pairs: MP51719-1

Matched pair protein standard: Ag0774

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 88328-3-PBS
Tested Reactivity human
Immunogen TMS1 fusion protein Ag0774
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name PYD and CARD domain containing
Synonyms
GenBank accession numberBC004470
Gene symbol ASC/TMS1
UniprotQ9ULZ3
Gene ID (NCBI) 29108
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 88328-2-PBS
Tested Reactivity human
Immunogen TMS1 fusion protein Ag0774
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name PYD and CARD domain containing
Synonyms
GenBank accession numberBC004470
Gene symbol ASC/TMS1
UniprotQ9ULZ3
Gene ID (NCBI) 29108
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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