验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17538-1-AP targets ARPC1A in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | ARPC1A fusion protein Ag11593 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin related protein 2/3 complex, subunit 1A, 41kDa |
| 别名 | Arc40, ARPC1A, SOP2 like protein, SOP2Hs, SOP2L |
| 计算分子量 | 370 aa, 42 kDa |
| 观测分子量 | 37-42 kDa |
| GenBank蛋白编号 | BC039594 |
| 基因名称 | ARPC1A |
| Gene ID (NCBI) | 10552 |
| RRID | AB_3085527 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92747 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARPC1A, also known as SOP2L, belongs to the WD repeat ARPC1 family. ARPC1A is most similar to ARPC1B, and the similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells (PMID: 9230079). ARPC1A functions as a component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks (PMID: 8978670). Western blot analysis detected ARPC1A at an apparent molecular mass of 37-42 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARPC1A antibody 17538-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |