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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84553-1-RR (same clone as 84553-1-PBS)

    WB analysis using 84553-1-RR (same clone as 84553-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84553-1-RR (ANP32CP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84553-1-PBS in a different storage buffer formulation.

  • IF Staining of LNCaP using 84553-1-RR (same clone as 84553-1-PBS)

    IF Staining of LNCaP using 84553-1-RR (same clone as 84553-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using ANP32CP antibody (84553-1-RR, Clone: 241562D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84553-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84553-1-RR

    Affinity and Kinetic Characterization of 84553-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84553-1-RR against Human ANP32CP were performed. The affinity constant is 7.76 nM.

ANP32CP Recombinant antibody, PBS Only

ANP32CP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84553-1-PBS

产品说明书

CloneNo. 241562D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

ANP32C, Tumorigenic protein pp32r1, PP32R1, 241562D12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84553-1-PBS targets ANP32CP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 ANP32CP fusion protein Ag35293 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acidic (leucine-rich) nuclear phosphoprotein 32 family, member C
别名 ANP32C, Tumorigenic protein pp32r1, PP32R1, 241562D12
计算分子量27 kDa
观测分子量27-30 kDa
GenBank蛋白编号NM_012403.1
基因名称 ANP32C/PP32R1
Gene ID (NCBI) 23520
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO43423
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ANP32CP (acidic nuclear phosphoprotein 32 family member C, pseudogene), also known as PP32R1. It is expected to be located in nucleus and perinuclear region of cytoplasm. Expressed in activated stem cells, such as mobilized CD34+ cells and cord blood CD34+ cells, but not in resting bone marrow CD34+ cells. Expressed in a variety of neoplastic cell lines, mainly in prostatic adenocarcinoma cell lines. Not expressed in normal prostatic tissue (PMID: 10086381). The calculated molecular weight of ANP32CP is 27 kDa. The protein could be the product of a pseudogene. Gene encoding ANP32CP is intronless suggesting that is pseudogene generated by retrotransposition.