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验证数据展示

  • IHC staining of human lung cancer using 84210-4-RR

    IHC staining of human lung cancer using 84210-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84210-4-RR (ANKRD6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 84210-4-RR

    IF Staining of A549 using 84210-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using ANKRD6 antibody (84210-4-RR, Clone: 241494D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84210-4-RR

    Affinity and Kinetic Characterization of 84210-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84210-4-RR against Human ANKRD6 were performed. The affinity constant is 0.722 nM.

ANKRD6 Recombinant monoclonal antibody

ANKRD6 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84210-4-RR

产品说明书

CloneNo. 241494D11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

241494D11, ANKRD 6, Ankyrin repeat domain-containing protein 6, Diversin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84210-4-RR targets ANKRD6 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19328

Product name: Recombinant human ANKRD6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-260 aa of BC042173

Sequence: MSQQDAVAALSERLLVAAYKGQTENVVQLINKGARVAVTKHGRTPLHLAANKGHLPVVQILLKAGCDLDVQDDGDQTALHRATVVGNTEIIAALIHEGCALDRQDKDGNTALHEASWHGFSQSAKLLVKAGANVLAKNKAGNTALHLACQNSHSQSTRVLLLAGSRADLKNNAGDTCLHVAARYNHLSIIRLLLTAFCSVHEKNQAGDTALHVAAALNHKKVAKILLEAGADTTIVNNAGQTPLETARYHNNPEVALLLT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ankyrin repeat domain 6
别名 241494D11, ANKRD 6, Ankyrin repeat domain-containing protein 6, Diversin
计算分子量 727 aa, 80 kDa
GenBank蛋白编号BC042173
基因名称 ANKRD6
Gene ID (NCBI) 22881
RRIDAB_3671767
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y2G4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for ANKRD6 antibody 84210-4-RRDownload protocol
IHC protocol for ANKRD6 antibody 84210-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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