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验证数据展示

  • IHC staining of mouse cerebellum using 83912-5-RR (same clone as 83912-5-PBS)

    IHC staining of mouse cerebellum using 83912-5-RR (same clone as 83912-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83912-5-RR (ANK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83912-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 83912-5-RR (same clone as 83912-5-PBS)

    IF Staining of mouse heart using 83912-5-RR (same clone as 83912-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (83912-5-RR, Clone: 240982G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83912-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 83912-5-RR (same clone as 83912-5-PBS)

    IF Staining of mouse heart using 83912-5-RR (same clone as 83912-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (83912-5-RR, Clone: 240982G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83912-5-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 83912-5-RR (same clone as 83912-5-PBS)

    FC experiment of HEK-293 using 83912-5-RR (same clone as 83912-5-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Ank2 Recombinant Antibody (83912-5-RR, Clone:240982G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83912-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83912-5-RR

    Affinity and Kinetic Characterization of 83912-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83912-5-RR against Human ANK2 were performed. The affinity constant is below 1 pM.

ANK2 Recombinant monoclonal antibody, PBS Only

ANK2 Uni-rAb® Recombinant Antibody for IHC, IF-P, FC (Intra), ELISA
Cat No. 83912-5-PBS

产品说明书

CloneNo. 240982G5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, FC (Intra), ELISA

别名

240982G5, ANK 2, Ankyrin 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83912-5-PBS targets ANK2 in IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34642

Product name: Recombinant human ANK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3571-3708 aa of NM_001148

Sequence: ERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGKHATDTNLVECLTKINRMDIVHLMETNTEPLQERISHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEQAVSKESETCDH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ankyrin 2, neuronal
别名 240982G5, ANK 2, Ankyrin 2
计算分子量434 kDa
观测分子量250-260 kDa
GenBank蛋白编号NM_001148
基因名称 ANK2
Gene ID (NCBI) 287
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ01484
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Ankyrins, belonging to the adapter protein family, consist of Ankyrin-R (ANK1), Ankyrin-B (ANK2), and Ankyrin-G (ANK3). Initially, ANK2 was considered as a key cytoskeletal-associated protein in the brain. Besides, it was discovered as a pivotal adaptor and scaffolding protein in the heart. Remarkably, recent studies have demonstrated that Ankyrins contribute to the modulation of various biological activities in cancers. (PMID: 36539782)

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