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验证数据展示

  • WB analysis using 81990-4-RR

    WB analysis using 81990-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81990-4-RR (Angiopoietin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 81990-4-RR

    IHC staining of mouse heart using 81990-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81990-4-RR (Angiopoietin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 81990-4-RR

    IHC staining of mouse heart using 81990-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81990-4-RR (Angiopoietin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse colon using 81990-4-RR

    IF Staining of mouse colon using 81990-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Angiopoietin 1 antibody (81990-4-RR, Clone: 240764D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse colon using 81990-4-RR

    IF Staining of mouse colon using 81990-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Angiopoietin 1 antibody (81990-4-RR, Clone: 240764D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 81990-4-RR

    FC experiment of HepG2 using 81990-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Angiopoietin 1 Recombinant antibody (81990-4-RR, Clone:240764D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 81990-4-RR

    Affinity and Kinetic Characterization of 81990-4-RR

    Biolayer interferometry (BLl) kinetic assays of 81990-4-RR against Human ANGPT1 were performed. The affinity constant is 6.37 nM.

Angiopoietin 1 Recombinant monoclonal antibody

Angiopoietin 1 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 81990-4-RR

产品说明书

CloneNo. 240764D10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, FC (Intra), ELISA

别名

ANGPT1, 240764D10, AGP1, AGPT, ANG 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, U-87 MG cells, K-562 cells, Raji cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue, human lung tissue
Positive FC (Intra) detected inHepG2 cells, A431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81990-4-RR targets Angiopoietin 1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18829

Product name: Recombinant human ANGPT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 161-293 aa of BC152411

Sequence: IQLLENSLSTYKLEKQLLQQTNEILKIHEKNSLLEHKILEMEGKHKEELDTLKEEKENLQGLVTRQTYIIQELEKQLNRATTNNSVLQKQQLELMDTVHNLVNLCTKEGVLLKGGKREEEKPFRDCADVYQAG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 angiopoietin 1
别名 ANGPT1, 240764D10, AGP1, AGPT, ANG 1
计算分子量 498 aa, 58 kDa
观测分子量70 kDa
GenBank蛋白编号BC152411
基因名称 Angiopoietin 1
Gene ID (NCBI) 284
RRIDAB_3670511
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ15389
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

实验方案

Product Specific Protocols
IHC protocol for Angiopoietin 1 antibody 81990-4-RRDownload protocol
WB protocol for Angiopoietin 1 antibody 81990-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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