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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 86518-1-RR (same clone as 86518-1-PBS)

    WB analysis using 86518-1-RR (same clone as 86518-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86518-1-RR (ALDH1A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86518-1-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 86518-1-RR (same clone as 86518-1-PBS)

    IF Staining of K-562 using 86518-1-RR (same clone as 86518-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ALDH1A2 antibody (86518-1-RR, Clone: 251371D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86518-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86518-1-RR

    Affinity and Kinetic Characterization of 86518-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86518-1-RR against Human ALDH1A2 were performed. The affinity constant is 0.179 nM.

    {{ptg:ProductFeaturesInfo}}

ALDH1A2 Recombinant monoclonal antibody, PBS Only

ALDH1A2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86518-1-PBS

产品说明书

CloneNo. 251371D10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

ALDH1A 2, EC:1.2.1.36, RALDH 2, RALDH(II), RalDH2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ALDH1A2 Ready-To-Use IHC Kit. ALDH1A2 primary antibody included.

产品信息

86518-1-PBS targets ALDH1A2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5062

Product name: Recombinant human ALDH1A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 181-481 aa of BC030589

Sequence: GQIIPWNFPLLMFAWKIAPALCCGNTVVIKPAEQTPLSALYMGALIKEVGKLIQEAAGRSNLKRVTLELGGKSPNIIFADADLDYAVEQAHQGVFFNQGQCCTAGSRIFVEESIYEEFVRRSVERAKRRVVGSPFDPTTEQGPQIDKKQYNKILELIQSGVAEGAKLECGGKGLGRKGFFIEPTVFSNVTDDMRIAKEEIFGPVQEILRFKTMDEVIERANNSDFGLVAAVFTNDINKALTVSSAMQAGTVWINCYNALNAQSPFGGFKMSGNGREMGEFGLREYSEVKTVTVKIPQKNS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aldehyde dehydrogenase 1 family, member A2
别名 ALDH1A 2, EC:1.2.1.36, RALDH 2, RALDH(II), RalDH2
计算分子量 518 aa, 57 kDa
观测分子量 53-57 kDa
GenBank蛋白编号BC030589
基因名称 ALDH1A2
Gene ID (NCBI) 8854
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO94788
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALDH1A2(aldehyde dehydrogenase family 1 member A2) is also named as RALDH2(retinal dehydrogenase 2) and belongs to the aldehyde dehydrogenase family. It is a cytosolic homotetramer(56.7 kDa subunits) exhibiting complex expression patterns throughout embryonic development. ALDH1A2 plays a role in retinoid metabolism during embryonic development where it is considered to be the major retinoic acid-synthesizing enzyme during early embryogenesis(PMID:20735668). Its expression is reduced in primary prostate tumors compared with normal prostate tissue suggested that it is normally expressed in the epithelial compartment of prostate tissue(PMID:16166285).It has 2 isoforms produced by alternative splicing.