验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293T cells, Jurkat cells, K-562 cells, NIH/3T3 cells, C2C12 cells, C6 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
86156-3-RR targets AIF in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | AIF fusion protein Ag13268 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | apoptosis-inducing factor, mitochondrion-associated, 1 |
别名 | AIFM1, Apoptosis-inducing factor 1, mitochondrial, EC:1.6.99.-, PDCD8 |
计算分子量 | 609 aa, 66 kDa |
观测分子量 | 67 kDa |
GenBank蛋白编号 | BC111065 |
基因名称 | AIF |
Gene ID (NCBI) | 9131 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | O95831 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).
实验方案
Product Specific Protocols | |
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WB protocol for AIF antibody 86156-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |