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验证数据展示

  • WB analysis using 83759-5-RR (same clone as 83759-5-PBS)

    WB analysis using 83759-5-RR (same clone as 83759-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83759-5-RR (RAGE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 83759-5-RR (same clone as 83759-5-PBS)

    IHC staining of mouse lung using 83759-5-RR (same clone as 83759-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83759-5-RR (RAGE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 83759-5-RR (same clone as 83759-5-PBS)

    IHC staining of mouse lung using 83759-5-RR (same clone as 83759-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83759-5-RR (RAGE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse lung using 83759-5-RR (same clone as 83759-5-PBS)

    IF Staining of mouse lung using 83759-5-RR (same clone as 83759-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using AGER/RAGE antibody (83759-5-RR, Clone: 240706C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • IHC staining of rat lung using 83759-5-RR (same clone as 83759-5-PBS)

    IHC staining of rat lung using 83759-5-RR (same clone as 83759-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83759-5-RR (AGER/RAGE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • IHC staining of rat lung using 83759-5-RR (same clone as 83759-5-PBS)

    IHC staining of rat lung using 83759-5-RR (same clone as 83759-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83759-5-RR (AGER/RAGE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse lung using 83759-5-RR (same clone as 83759-5-PBS)

    IF Staining of mouse lung using 83759-5-RR (same clone as 83759-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using AGER/RAGE antibody (83759-5-RR, Clone: 240706C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83759-5-RR

    Affinity and Kinetic Characterization of 83759-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83759-5-RR against Rat AGER/RAGE were performed. The affinity constant is 3.46 nM.

Rat AGER/RAGE Recombinant monoclonal antibody, PBS Only

AGER/RAGE Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 83759-5-PBS

产品说明书

CloneNo. 240706C3

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

AGER, RAGE, 240706C3, Advanced glycosylation end product-specific receptor

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83759-5-PBS targets AGER/RAGE in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg1135

Product name: Recombinant Rat AGER/RAGE protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 23-341 aa of

Sequence: GQNITARIGEPLMLSCKGAPKKPTQKLEWKLNTGRTEAWKVLSPQGDPWDSVARILPNGSLLLPAIGIVDEGTFRCRATNRLGKEVKSNYRVRVYQIPGKPEIVNPASELTANVPNKVGTCVSEGSYPAGTLSWHLDGKPLIPDGKGTVVKEETRRHPETGLFTLRSELTVTPAQGGTTPTYSCSFSLGLPRRRPLNTAPIQPRVREPLPPEGIQLLVEPEGGTVAPGGTVTLTCAISAQPPPQIHWIKDGTPLPLAPSPVLLLPEVGHEDEGIYSCVATHPSHGPQESPPVNIRVTETGDEGQAAGSVDGSGLGTLAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 advanced glycosylation end product-specific receptor
别名 AGER, RAGE, 240706C3, Advanced glycosylation end product-specific receptor
计算分子量43 kDa
观测分子量43 kDa
基因名称 Ager
Gene ID (NCBI) 81722
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ63495
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a member of the immunoglobulin superfamily of cell surface receptors, which interacts with distinct families of ligands, mediating diverse functions in a broad array of cell types including cellular migration, proliferation, survival and apoptosis (PMID: 12645002; 17425919). It senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, phospholipids and glycosaminoglycans (PMID: 19910580; 28627626). It interacts with distinct molecules implicated in homeostasis, development, inflammation, and certain diseases such as diabetes and Alzheimer's disease (PMID: 26253613; 31079281).

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