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验证数据展示

  • WB analysis using 84443-1-RR

    WB analysis using 84443-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84443-1-RR (ADAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293T using 84443-1-RR

    WB analysis of HEK-293T using 84443-1-RR

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 84443-1-RR (ADAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84443-1-RR

    IHC staining of mouse brain using 84443-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84443-1-RR (ADAP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 84443-1-RR

    IHC staining of mouse brain using 84443-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84443-1-RR (ADAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ADAP1 Recombinant antibody

ADAP1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84443-1-RR

产品说明书

CloneNo. 241370D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

241370D6, Arf-GAP with dual PH domain-containing protein 1, ArfGAP with dual PH domains 1, CENTA1, Centaurin alpha 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HEK-293T cells, mouse small intestine tissue, rat kidney tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84443-1-RR targets ADAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ADAP1 fusion protein Ag4898 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ArfGAP with dual PH domains 1
别名 241370D6, Arf-GAP with dual PH domain-containing protein 1, ArfGAP with dual PH domains 1, CENTA1, Centaurin alpha 1
计算分子量 374 aa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC033747
基因名称 ADAP1
Gene ID (NCBI) 11033
RRIDAB_3671968
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO75689
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADAP1 antibody 84443-1-RRDownload protocol
IHC protocol for ADAP1 antibody 84443-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols