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验证数据展示

  • WB analysis using 32970-1-AP

    WB analysis using 32970-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32970-1-AP (ADAM11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 32970-1-AP

    IHC staining of mouse lung using 32970-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 32970-1-AP (ADAM11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 32970-1-AP

    IF Staining of NIH/3T3 using 32970-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ADAM11 antibody (32970-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

ADAM11 Polyclonal antibody

ADAM11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 32970-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ADAM 11, Disintegrin and metalloproteinase domain-containing protein 11, MDC, Metalloproteinase-like, disintegrin-like, and cysteine-rich protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat brain tissue, rat cerebellum tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32970-1-AP targets ADAM11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35877

Product name: Recombinant human ADAM11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 150-300 aa of NM_001318933

Sequence: STCQGLHGVFSDGNLTYIVEPQEVAGPWGAPQGPLPHLIYRTPLLPDPLGCREPGCLFAVPAQSAPPNRPRLRRKRQVRRGHPTVHSETKYVELIVINDHQLFEQMRQSVVLTSNFAKSVVNLADVIYKEQLNTRIVLVAMETWADGDKIQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADAM metallopeptidase domain 11
别名 ADAM 11, Disintegrin and metalloproteinase domain-containing protein 11, MDC, Metalloproteinase-like, disintegrin-like, and cysteine-rich protein
计算分子量83kDa
观测分子量83 kDa
GenBank蛋白编号NM_001318933
基因名称 ADAM11
Gene ID (NCBI) 4185
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO75078
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADAM11 (A Disintegrin And Metalloprotease 11) is a non-catalytic, transmembrane member of the ADAM family. Although it retains the prototypical multi-domain architecture (signal peptide ‑ pro‑domain ‑ metalloprotease-like ‑ disintegrin ‑ cysteine-rich ‑ EGF-like ‑ TM ‑ cytoplasmic tail), it lacks the consensus HEXGHXXGXXHD zinc-binding motif, rendering it proteolytically inactive . Instead, ADAM11 functions as a cell-adhesion and signalling scaffold. (PMID: 31236626)

实验方案

Product Specific Protocols
IF protocol for ADAM11 antibody 32970-1-APDownload protocol
IHC protocol for ADAM11 antibody 32970-1-APDownload protocol
WB protocol for ADAM11 antibody 32970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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