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ACIN1 Polyclonal antibody

ACIN1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 23937-1-AP

Print datasheet

Synonyms

ACIN1, ACINUS, ACN, fSAP152, KIAA0670

验证数据展示

  • WB analysis using 23937-1-AP

    WB analysis using 23937-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23937-1-AP (ACIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 23937-1-AP

    IHC staining of mouse cerebellum using 23937-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 23937-1-AP (ACIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23937-1-AP targets ACIN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen ACIN1 fusion protein Ag21036 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apoptotic chromatin condensation inducer 1
Synonyms ACIN1, ACINUS, ACN, fSAP152, KIAA0670
Calculated Molecular Weight 1341 aa, 152 kDa
Observed Molecular Weight250 kDa, 80-85 kDa
GenBank Accession NumberBC140805
Gene Symbol ACIN1
Gene ID (NCBI) 22985
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKV3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. ACIN1 is a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. There are three isoforms of human acinus (the L, S and S' forms) shows at about 80-85 kDa and 220-250 kDa by Western Blot, which were probably generated by alternative splicing (PMID: 10490026). 23937-1-AP can detect all three isoforms of ACIN1. Another antibody 30522-1-AP specificly targets at the ACIN1-L.

实验方案

Product Specific Protocols
WB protocol for ACIN1 antibody 23937-1-APDownload protocol
IHC protocol for ACIN1 antibody 23937-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols