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验证数据展示

  • WB analysis using 25812-1-AP

    WB analysis using 25812-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25812-1-AP (A1CF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 25812-1-AP

    IHC staining of mouse colon using 25812-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25812-1-AP (A1CF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 25812-1-AP

    IHC staining of mouse colon using 25812-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25812-1-AP (A1CF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

A1CF Polyclonal antibody

A1CF Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25812-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

APOBEC1-stimulating protein, APOBEC1CF, APOBEC1 complementation factor, ACF65, ACF64

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25812-1-AP targets A1CF in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原

CatNo: Ag22929

Product name: Recombinant human A1CF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-122 aa of BC054873

Sequence: MESNHKSGDGLSGTQKEAALRALVQRTGYSLVQENGQRKYGGPPPGWDAAPPERGCEIFIGKLPRDLFEDELIPLCEKIGKIYEMRMMMDFNGNNRGYAFVTFSNKVEAKNAIKQLNNYEIR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 APOBEC1 complementation factor
别名 APOBEC1-stimulating protein, APOBEC1CF, APOBEC1 complementation factor, ACF65, ACF64
计算分子量 594 aa, 65 kDa
观测分子量65 kDa
GenBank蛋白编号BC054873
基因名称 A1CF
Gene ID (NCBI) 29974
RRIDAB_3669501
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ94
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

A1CF, also known as Apobec-1 complementation factor, is an RNA-binding protein that plays a crucial role in the post-transcriptional editing of apolipoprotein B (apoB) mRNA. It is a component of the enzyme complex responsible for converting a CAA codon for glutamine to a UAA stop codon in apoB mRNA, resulting in the production of a truncated protein, apoB48, instead of the full-length apoB100.

实验方案

Product Specific Protocols
WB protocol for A1CF antibody 25812-1-APDownload protocol
IHC protocol for A1CF antibody 25812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Int Immunopharmacol

Metformin attenuated sepsis-related liver injury by modulating the DNA methylation and hydroxymethylation landscape

Authors - Fengyu Liu
View Article