验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, Raji cells, K-562 cells, HEK-293T cells |
| Positive IF/ICC detected in | Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84185-5-RR targets 4-1BBL/TNFSF9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Fusion Protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor (ligand) superfamily, member 9 |
| 别名 | TNFSF9, CD137L, 4-1BB ligand, 4 1BBL, 4 1BB ligand |
| 计算分子量 | 254 aa, 27 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC104805 |
| 基因名称 | TNFSF9 |
| Gene ID (NCBI) | 8744 |
| RRID | AB_3671740 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P41273 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNFSF9, also named as 4-1BBL, belongs to the tumor necrosis factor family. It is a cytokine that binds to TNFRSF9. TNSF9 induces the proliferation of activated peripheral blood T-cells. It may have a role in activation-induced cell death (AICD) and may play a role in cognate interactions between T-cells and B-cells/macrophages.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for 4-1BBL/TNFSF9 antibody 84185-5-RR | Download protocol |
| WB protocol for 4-1BBL/TNFSF9 antibody 84185-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
