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SLC12A1/NKCC2 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.156-10 ng/mL

Cat no : MP03191-1

Synonyms

NKCC2, SLC12A1, BSC1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter

Validation Data Gallery

  • Sandwich ELISA standard curve of MP03191-1, Human SLC12A1/NKCC2 Recombinant Matched Antibody Pair - PBS only. 87210-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5325. 87210-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

    Sandwich ELISA standard curve of MP03191-1

    Sandwich ELISA standard curve of MP03191-1, Human SLC12A1/NKCC2 Recombinant Matched Antibody Pair - PBS only. 87210-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5325. 87210-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • The mean SLC12A1/NKCC2 concentration was determined to be 51.78 ng/mL in mouse kidney tissue extract based on a 8.40 mg/mL extract load, 13.25 ng/mL in mouse heart tissue extract based on a 5.40 mg/mL extract load, 18.62 ng/mL in mouse liver tissue extract based on a 8.40 mg/mL extract load and 9.84 ng/mL in mouse spleen tissue extract based on a 6.40 mg/mL extract load.

    Sandwich ELISA sample test of MP03191-1

    The mean SLC12A1/NKCC2 concentration was determined to be 51.78 ng/mL in mouse kidney tissue extract based on a 8.40 mg/mL extract load, 13.25 ng/mL in mouse heart tissue extract based on a 5.40 mg/mL extract load, 18.62 ng/mL in mouse liver tissue extract based on a 8.40 mg/mL extract load and 9.84 ng/mL in mouse spleen tissue extract based on a 6.40 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP03191-1 targets SLC12A1/NKCC2 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.156-10 ng/mL (Sandwich ELISA);

Capture antibody: SLC12A1 Recombinant monoclonal antibody, PBS Only (Capture) 87210-3-PBS (252363G10). 100 μg. Concentration 1 mg/ml.

Detection antibody: SLC12A1 Recombinant monoclonal antibody, PBS Only (Detector) 87210-2-PBS (252363A10). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Ag5325

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 87210-3-PBS
Tested Reactivity human
Immunogen SLC12A1 fusion protein Ag5325
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 12 (sodium/potassium/chloride transporters), member 1
Synonyms NKCC2, BSC1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter
GenBank accession numberBC040138
Gene symbol SLC12A1/NKCC2
UniprotQ13621
Gene ID (NCBI) 6557
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 87210-2-PBS
Tested Reactivity human
Immunogen SLC12A1 fusion protein Ag5325
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 12 (sodium/potassium/chloride transporters), member 1
Synonyms NKCC2, BSC1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter
GenBank accession numberBC040138
Gene symbol SLC12A1/NKCC2
UniprotQ13621
Gene ID (NCBI) 6557
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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