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RRN3 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human, mouse

Applications

Sandwich ELISA

Range

Sandwich ELISA: 156-5000 pg/mL

Cat no : MP02997-1

Synonyms

RNA polymerase I-specific transcription initiation factor RRN3, TIF IA, TIFIA, TIF-IA

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02997-1, Human RRN3 Recombinant Matched Antibody Pair - PBS only. 87046-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag23207. 87046-1-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL.

    Sandwich ELISA standard curve of MP02997-1

    Sandwich ELISA standard curve of MP02997-1, Human RRN3 Recombinant Matched Antibody Pair - PBS only. 87046-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag23207. 87046-1-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL.

  • The mean RRN3 concentration was determined to be 120.4 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 99.7 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 54.5 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 51.8 ng/mL in A549 cell extract based on a 1.2 mg/mL extract load, 27.1 ng/mL in HCT 116 extract based on a 1.2 mg/mL extract load and 76.5 ng/mL in HuH-7 cell extract based on a 1.2 mg/mL extract load.

    Sandwich ELISA sample test of MP02997-1

    The mean RRN3 concentration was determined to be 120.4 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 99.7 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 54.5 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 51.8 ng/mL in A549 cell extract based on a 1.2 mg/mL extract load, 27.1 ng/mL in HCT 116 extract based on a 1.2 mg/mL extract load and 76.5 ng/mL in HuH-7 cell extract based on a 1.2 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP02997-1 targets RRN3 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 156-5000 pg/mL (Sandwich ELISA);

Capture antibody: RRN3 Recombinant monoclonal antibody, PBS Only (Capture) 87046-2-PBS (252129D5). 100 μg. Concentration 1 mg/ml.

Detection antibody: RRN3 Recombinant monoclonal antibody, PBS Only (Detector) 87046-1-PBS (252129D11). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Ag23207

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 87046-2-PBS
Tested Reactivity human
Immunogen RRN3 fusion protein Ag23207
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)
Synonyms
GenBank accession numberBC132688
Gene symbol RRN3
UniprotQ9NYV6
Gene ID (NCBI) 54700
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 87046-1-PBS
Tested Reactivity human, mouse
Immunogen RRN3 fusion protein Ag23207
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)
Synonyms RNA polymerase I-specific transcription initiation factor RRN3, TIF IA, TIFIA, TIF-IA
GenBank accession numberBC132688
Gene symbol RRN3
UniprotQ9NYV6
Gene ID (NCBI) 54700
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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