验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293T cells, BGC-823 cells, K-562 cells, HuH-7 cells, HGC-27 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86857-1-RR targets FBXL13 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag10903 Product name: Recombinant human FBXL13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 358-707 aa of BC031285 Sequence: NDMPTLTDNCVKALVEKCSRITSLVFTGAPHISDCTFRALSACKLRKIRFEGNKRVTDASFKFIDKNYPNLSHIYMADCKGITDSSLRSLSPLKQLTVLNLANCVRIGDMGLKQFLDGPASMRIRELNLSNCVRLSDASVMKLSERCPNLNYLSLRNCEHLTAQGIGYIVNIFSLVSIDLSGTDISNEAFCKSSLILEHLDVSYCSQLSDMIIKALAIYCINLTSLSIAGCPKITDSAMEMLSAKCHYLHILDISGCVLLTDQILEDLQIGCKQLRILKMQYCTNISKKAAQRMSSKVQQQEYNTNGPPRWFGYDREGNPVTELDNITSSKGALELTVKKSTYSSEDQAA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | F-box and leucine-rich repeat protein 13 |
| 别名 | DRC6, Dynein regulatory complex subunit 6, FBL13, F-box and leucine-rich repeat protein 13, F-box/LRR-repeat protein 13 |
| 计算分子量 | 707 aa, 81 kDa |
| 观测分子量 | 84 kDa |
| GenBank蛋白编号 | BC031285 |
| 基因名称 | FBXL13 |
| Gene ID (NCBI) | 222235 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8NEE6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBXL13, also known as F-box and leucine-rich repeat protein 13, is a human protein-coding gene located on chromosome 7q22.1. It is a member of the F-box protein family, characterized by an approximately 40-amino acid F-box motif. FBXL13 is part of the SCF-type E3 ubiquitin ligase complex, which plays a role in protein ubiquitination and degradation. The gene has multiple isoforms, including 84 kDa, 81 kDa, 79 kDa, and 53 kDa variants. While its specific function within the complex is not fully understood, FBXL13 has been implicated in regulating centrosome homeostasis and cell migration by directing the proteolysis of CEP192. Additionally, it may play a role in circadian rhythms by ubiquitinating and mediating the degradation of cryptochrome proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXL13 antibody 86857-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
