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验证数据展示

  • WB analysis using 86549-1-RR (same clone as 86549-1-PBS)

    WB analysis using 86549-1-RR (same clone as 86549-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86549-1-RR (CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86549-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86549-1-RR (same clone as 86549-1-PBS)

    IF Staining of HepG2 using 86549-1-RR (same clone as 86549-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CS antibody (86549-1-RR, Clone: 251433D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86549-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse heart using 86549-1-RR (same clone as 86549-1-PBS)

    WB analysis of mouse heart using 86549-1-RR (same clone as 86549-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 86549-1-RR (CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86549-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 86549-1-RR (same clone as 86549-1-PBS)

    IF Staining of U2OS using 86549-1-RR (same clone as 86549-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CS antibody (86549-1-RR, Clone: 251433D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86549-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02513-1

    Cytometric bead array sample test of MP02513-1

    The mean Citrate synthase concentration was determined to be 135.1 ng/mL in HepG2 cell extract based on a 1.3 mg/mL extract load.

  • Cytometric bead array standard curve of MP02513-1

    Cytometric bead array standard curve of MP02513-1

    Cytometric bead array standard curve of MP02513-1, Citrate synthase Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86549-2-PBS. Detection antibody: 86549-1-PBS. Standard: Ag9117. Range: 6.25-200 ng/mL.

  • Affinity and Kinetic Characterization of 86549-1-RR

    Affinity and Kinetic Characterization of 86549-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86549-1-RR against Human Citrate synthase were performed. The affinity constant is 0.995 nM.

Citrate synthase Recombinant monoclonal antibody, PBS Only (Detector)

Citrate synthase Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86549-1-PBS

产品说明书

CloneNo. 251433D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

CS, CISY, Citrate, Citrate synthase, mitochondrial, EC:2.3.3.1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CS Ready-To-Use IHC Kit. CS primary antibody included.

产品信息

86549-1-PBS targets Citrate synthase as part of a matched antibody pair:

MP02513-1: 86549-2-PBS capture and 86549-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9117

Product name: Recombinant human CS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 132-466 aa of BC010106

Sequence: EQVSWLSKEWAKRAALPSHVVTMLDNFPTNLHPMSQLSAAVTALNSESNFARAYAQGISRTKYWELIYEDSMDLIAKLPCVAAKIYRNLYREGSGIGAIDSNLDWSHNFTNMLGYTDHQFTELTRLYLTIHSDHEGGNVSAHTSHLVGSALSDPYLSFAAAMNGLAGPLHGLANQEVLVWLTQLQKEVGKDVSDEKLRDYIWNTLNSGRVVPGYGHAVLRKTDPRYTCQREFALKHLPNDPMFKLVAQLYKIVPNVLLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALGFPLERPKSMSTEGLMKFVDSKSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 citrate synthase
别名 CS, CISY, Citrate, Citrate synthase, mitochondrial, EC:2.3.3.1
计算分子量 466 aa, 52 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC010106
基因名称 Citrate Synthase
Gene ID (NCBI) 1431
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75390
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038).

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