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验证数据展示

  • WB analysis of HL-60 using 86432-1-RR

    WB analysis of HL-60 using 86432-1-RR

    HL-60 cells were subjected to SDS PAGE followed by western blot with 86432-1-RR (CEBPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 86432-1-RR

    WB analysis of Jurkat using 86432-1-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 86432-1-RR (CEBPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 86432-1-RR

    IF Staining of HepG2 using 86432-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBPE antibody (86432-1-RR, Clone: 251303B10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 86432-1-RR

    IF Staining of HeLa using 86432-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CEBPE antibody (86432-1-RR, Clone: 251303B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A431 using 86432-1-RR

    IF Staining of A431 using 86432-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CEBPE antibody (86432-1-RR, Clone: 251303B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CEBPE Recombinant monoclonal antibody

CEBPE Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86432-1-RR

产品说明书

CloneNo. 251303B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

C/EBP epsilon, CCAAT/enhancer-binding protein epsilon, CRP1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HL-60 cells
Positive IF/ICC detected inHepG2 cells, A431 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86432-1-RR targets CEBPE in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5589

Product name: Recombinant human CEBPE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-281 aa of BC035797

Sequence: MSHGTYYECEPRGGQQPLEFSGGRAGPGELGDMCEHEASIDLSAYIESGEEQLLSDLFAVKPAPEARGLKGPGTPAFPHYLPPDPRPFAYPPHTFGPDRKALGPGIYSSPGSYDPRAVAVKEEPRGPEGSRAASRGSYNPLQYQVAHCGQTAMHLPPTLAAPGQPLRVLKAPLATAAPPCSPLLKAPSPAGPLHKGKKAVNKDSLEYRLRRERNNIAVRKSRDKAKRRILETQQKVLEYMAENERLRSRVEQLTQELDTLRNLFRQIPEAANLIKGVGGCS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CCAAT/enhancer binding protein (C/EBP), epsilon
别名 C/EBP epsilon, CCAAT/enhancer-binding protein epsilon, CRP1
计算分子量 31 kDa
观测分子量 31-38 kDa
GenBank蛋白编号BC035797
基因名称 CEBPE
Gene ID (NCBI) 1053
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15744
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEBPE (CCAAT/enhancer binding protein epsilon), also known as C/EBP-epsilon and CRP1, is a transcription factor involved in late myeloid lineage differentiation and cellular function (PMID: 31201888). CEBPE is expressed in a stage-specific manner during myeloid differentiation and regulates transition from the promyelocyte to the myelocyte (PMID: 29773596). Depletion of CEBPE in Acute lymphoblastic leukaemia (ALL) cells reduces cell growth, correspondingly CEBPE binds to the promoters of electron transport and energy generation genes (PMID: 29977016).

实验方案

Product Specific Protocols
IF protocol for CEBPE antibody 86432-1-RRDownload protocol
WB protocol for CEBPE antibody 86432-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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