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验证数据展示

  • WB analysis of mouse liver using 86415-1-RR

    WB analysis of mouse liver using 86415-1-RR

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 86415-1-RR (AMDHD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86415-1-RR

    WB analysis using 86415-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86415-1-RR (AMDHD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86415-1-RR

    Affinity and Kinetic Characterization of 86415-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86415-1-RR against Human AMDHD1 were performed. The affinity constant is 9.59 nM.

    {{ptg:ProductFeaturesInfo}}

AMDHD1 Recombinant monoclonal antibody

AMDHD1 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86415-1-RR

产品说明书

CloneNo. 250819A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Amidohydrolase domain-containing protein 1, EC:3.5.2.7, HMFT1272

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86415-1-RR targets AMDHD1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15420

Product name: Recombinant human AMDHD1 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 78-426 aa of BC029146

Sequence: CILPGLVDAHTHPVWAGERVHEFAMKLAGATYMEIHQAGGGIHFTVERTRQATEEELFRSLQQRLQCMMRAGTTLVECKSGYGLDLETELKMLRVIERARRELDIGISATYCGAHSVPKGKTATEAADDIINNHLPKLKELGRNGEIHVDNIDVFCEKGVFDLDSTRRILQRGKDIGLQINFHGDELHPMKAAELGAELGAQAISHLEEVSDEGIVAMATARCSAILLPTTAYMLRLKQPRARKMLDEGVIVALGSDFNPNAYCFSMPMVMHLACVNMRMSMPEALAAATINAAYALGKSHTHGSLEVGKQGDLIIINSSRWEHLIYQFGGHHELIEYVIAKGKLIYKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 amidohydrolase domain containing 1
别名 Amidohydrolase domain-containing protein 1, EC:3.5.2.7, HMFT1272
计算分子量 426 aa, 47 kDa
观测分子量45-50 kDa
GenBank蛋白编号BC029146
基因名称 AMDHD1
Gene ID (NCBI) 144193
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96NU7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMDHD1 is a protein-coding gene that encodes the enzyme imidazolonepropionase. It is involved in the histidine catabolic process and has one potential isoform. The protein of AMDHD1 is located in the cytoplasm, and is expressed in all tissues, mainly in the liver, kidney and adrenal gland.

实验方案

Product Specific Protocols
WB protocol for AMDHD1 antibody 86415-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols