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验证数据展示

  • WB analysis using 86269-3-RR

    WB analysis using 86269-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86269-3-RR (CD107b / LAMP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocellular cancer using 86269-3-RR

    IHC staining of human hepatocellular cancer using 86269-3-RR

    Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86269-3-RR (CD107b / LAMP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LAMP2 antibody included
  • IHC staining of human hepatocellular cancer using 86269-3-RR

    IHC staining of human hepatocellular cancer using 86269-3-RR

    Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86269-3-RR (CD107b / LAMP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LAMP2 antibody included
  • IF Staining of U-251 using 86269-3-RR

    IF Staining of U-251 using 86269-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using LAMP2/ CD107b antibody (86269-3-RR, Clone: 250948D7 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CD107b / LAMP2 Recombinant monoclonal antibody

CD107b / LAMP2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86269-3-RR

产品说明书

CloneNo. 250948D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CD107b, LAMP2, CD107 antigen-like family member B, LAMP 2, LAMP-2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHUVEC cells, Jurkat cells, HepG2 cells, L02 cells, U-251 cells, Caki-2 cells, A-253 cells
Positive IHC detected inhuman hepatocellular cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells
Planning an IHC experiment? We recommend our IHCeasy LAMP2 Ready-To-Use IHC Kit. LAMP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86269-3-RR targets CD107b / LAMP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg4998

Product name: Recombinant Human LAMP2/ CD107b protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 29-375 aa of NM_002294.3

Sequence: LELNLTDSENATCLYAKWQMNFTVRYETTNKTYKTVTISDHGTVTYNGSICGDDQNGPKIAVQFGPGFSWIANFTKAASTYSIDSVSFSYNTGDNTTFPDAEDKGILTVDELLAIRIPLNDLFRCNSLSTLEKNDVVQHYWDVLVQAFVQNGTVSTNEFLCDKDKTSTVAPTIHTTVPSPTTTPTPKEKPEAGTYSVNNGNDTCLLATMGLQLNITQDKVASVININPNTTHSTGSCRSHTALLRLNSSTIKYLDFVFAVKNENRFYLKEVNISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDLRVQPFNVTQGKYSTAQDCSADDDNF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lysosomal-associated membrane protein 2
别名 CD107b, LAMP2, CD107 antigen-like family member B, LAMP 2, LAMP-2
计算分子量45 kDa
观测分子量100-130 kDa
GenBank蛋白编号NM_002294.3
基因名称 LAMP2
Gene ID (NCBI) 3920
ENSEMBL Gene IDENSG00000005893
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13473-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

实验方案

Product Specific Protocols
IF protocol for CD107b / LAMP2 antibody 86269-3-RRDownload protocol
IHC protocol for CD107b / LAMP2 antibody 86269-3-RRDownload protocol
WB protocol for CD107b / LAMP2 antibody 86269-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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