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验证数据展示

  • WB analysis using 86176-1-RR

    WB analysis using 86176-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86176-1-RR (BRD7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 86176-1-RR

    IF Staining of U2OS using 86176-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BRD7 antibody (86176-1-RR, Clone: 250776E3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 86176-1-RR

    IF Staining of HeLa using 86176-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BRD7 antibody (86176-1-RR, Clone: 250776E3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

BRD7 Recombinant monoclonal antibody

BRD7 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86176-1-RR

产品说明书

CloneNo. 250776E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

BP75, Bromodomain-containing protein 7, CELTIX1, NAG4, Protein CELTIX 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, SH-SY5Y cells, COLO 320 cells
Planning an IHC experiment? We recommend our IHCeasy BRD7 Ready-To-Use IHC Kit. BRD7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86176-1-RR targets BRD7 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1461

Product name: Recombinant human BRD7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-353 aa of BC050728

Sequence: MGKKHKKHKSDKHLYEEYVEKPLKLVLKVGGNEVTELSTGSSGHDSSLFEDKNDHDKHKDRKRKKRKKGEKQIPGEEKGRKRRRVKEDKKKRDRDRVENEAEKDLQCHAPVRLDLPPEKPLTSSLAKQEEVEQTPLQEALNQLMRQLQRKDPSAFFSFPVTDFIAPGYSMIIKHPMDFSTMKEKIKNNDYQSIEELKDNFKLMCTNAMIYNKPETIYYKAAKKLLHSGMKILSQERIQSLKQSIDFMADLQKTRKQKDGTDTSQSGEDGGCWQREREDSGDAEAHAFKSPSKENKKKDKDMLEDKFKSNNLEREQEQLDRIVKESGGKLTRRLVNSQCEFERRKPDGTTTLGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 bromodomain containing 7
别名 BP75, Bromodomain-containing protein 7, CELTIX1, NAG4, Protein CELTIX 1
计算分子量 651 aa, 74 kDa
观测分子量74-78 kDa
GenBank蛋白编号BC050728
基因名称 BRD7
Gene ID (NCBI) 29117
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NPI1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner.

实验方案

Product Specific Protocols
WB protocol for BRD7 antibody 86176-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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