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验证数据展示

  • WB analysis using 86065-1-RR

    WB analysis using 86065-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86065-1-RR (ZNF74 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86065-1-RR

    WB analysis using 86065-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86065-1-RR (ZNF74 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86065-1-RR

    Affinity and Kinetic Characterization of 86065-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86065-1-RR against Human ZNF74 were performed. The affinity constant is below 1 pM.

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ZNF74 Recombinant monoclonal antibody

ZNF74 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86065-1-RR

产品说明书

CloneNo. 250627F12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Cos52, hZNF7, Zfp520, Zinc finger protein 520, Zinc finger protein 74

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, human skeletal muscle tissue, rat skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86065-1-RR targets ZNF74 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13580

Product name: Recombinant human ZNF74 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 464-644 aa of BC013395

Sequence: RIHSGEKPFKCNECGKAFSSHAYLIVHRRIHTGEKPFDCSQCWKAFSCHSSLIVHQRIHTGEKPYKCSECGRAFSQNHCLIKHQKIHSGEKSFKCEKCGEMFNWSSHLTEHQRLHSEGKPLAIQFNKHLLSTYYVPGSLLGAGDAGLRDVDPIDALDVAKLLCVVPPRAGRNFSLGSKPRN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger protein 74
别名 Cos52, hZNF7, Zfp520, Zinc finger protein 520, Zinc finger protein 74
计算分子量 644 aa, 72 kDa
观测分子量64-72 kDa
GenBank蛋白编号BC013395
基因名称 ZNF74
Gene ID (NCBI) 7625
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16587
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF74 antibody 86065-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols