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验证数据展示

  • WB analysis using 85917-1-RR

    WB analysis using 85917-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85917-1-RR (Cd37 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 85917-1-RR

    IHC staining of mouse spleen using 85917-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85917-1-RR (CD37 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 85917-1-RR

    IHC staining of mouse spleen using 85917-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85917-1-RR (CD37 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A20 using 85917-1-RR

    IF Staining of A20 using 85917-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A20 cells using Cd37 antibody (85917-1-RR, Clone: 250262A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of mouse spleen using 85917-1-RR

    IF Staining of mouse spleen using 85917-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD37 antibody (85917-1-RR, Clone: 250262A3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat spleen using 85917-1-RR

    IF Staining of rat spleen using 85917-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD37 antibody (85917-1-RR, Clone: 250262A3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD37 Recombinant monoclonal antibody

CD37 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 85917-1-RR

产品说明书

CloneNo. 250262A3

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

Leukocyte antigen CD37, Tetraspanin 26, TSPAN26

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue, rat spleen tissue, A20 cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat spleen tissue, mouse spleen tissue
Positive IF/ICC detected inA20 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85917-1-RR targets CD37 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg2729

Product name: Recombinant Mouse CD37 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 112-241 aa of NM_007645.4

Sequence: RVRLERRVQELVLRTIQSYRTNPDETAAEESWDYAQFQLRCCGWQSPRDWNKAQMLKANESEEPFVPCSCYNSTATNDSTVFDKLFFSQLSRLGPRAKLRQTADICALPAKAHIYREGCAQSLQKWLHNN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD37 antigen
别名 Leukocyte antigen CD37, Tetraspanin 26, TSPAN26
计算分子量32 kDa
观测分子量45 kDa
GenBank蛋白编号NM_007645.4
基因名称 Cd37
Gene ID (NCBI) 12493
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ61470
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).

实验方案

Product Specific Protocols
IF protocol for CD37 antibody 85917-1-RRDownload protocol
IHC protocol for CD37 antibody 85917-1-RRDownload protocol
WB protocol for CD37 antibody 85917-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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