Various lysates were subjected to SDS PAGE followed by western blot with 85802-1-RR (CD74 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85802-1-PBS in a different storage buffer formulation.

Sandwich ELISA standard curve of MP02137-1, Mouse CD74 Recombinant Matched Antibody Pair - PBS only. 85802-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2716. 85802-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

RAW 264.7 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD74, and measured 14.17 ng/mL.

The mean CD74 concentration was determined to be 856.01 ng/mL in mouse thymus tissue extract based on a 5.80 mg/mL extract load and 789.39 ng/mL in mouse spleen tissue extract based on a 8.50 mg/mL extract load.

Serum of sixteen mice was measured. The CD74 concentration of detected samples was determined to be 21.51 pg/mL with a range of 12.61-42.94 pg/mL.

Cytometric bead array standard curve of MP02137-1, MOUSE CD74 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85802-1-PBS. Detection antibody: 85802-2-PBS. Standard: Eg2716. Range: 0.781-50 ng/mL.