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验证数据展示

  • WB analysis using 84933-5-RR

    WB analysis using 84933-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84933-5-RR (YOD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 84933-5-RR

    IF Staining of K-562 using 84933-5-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using YOD1 antibody (84933-5-RR, Clone: 242498G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84933-5-RR

    Affinity and Kinetic Characterization of 84933-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84933-5-RR against Human YOD1 were performed. The affinity constant is 1.07 nM.

YOD1 Recombinant monoclonal antibody

YOD1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84933-5-RR

产品说明书

CloneNo. 242498G6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

242498G6, DUBA 8, DUBA8, DUBA-8, EC:3.4.19.12

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells, HEK-293 cells, HeLa cells
Positive IF/ICC detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84933-5-RR targets YOD1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21844

Product name: Recombinant human YOD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 63-348 aa of BC137167

Sequence: LSSRTRVRELQGQIAAITGIAPGGQRILVGYPPECLDLSNGDTILEDLPIQSGDMLIIEEDQTRPRSSPAFTKRGASSYVRETLPVLTRTVVPADNSCLFTSVYYVVEGGVLNPACAPEMRRLIAQIVASDPDFYSEAILGKTNQEYCDWIKRDDTWGGAIEISILSKFYQCEICVVDTQTVRIDRFGEDAGYTKRVLLIYDGIHYDPLQRNFPDPDTPPLTIFSSNDDIVLVQALELADEARRRRQFTDVNRFTLRCMVCQKGLTGQAEAREHAKETGHTNFGEV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae)
别名 242498G6, DUBA 8, DUBA8, DUBA-8, EC:3.4.19.12
计算分子量 348 aa, 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC137167
基因名称 YOD1
Gene ID (NCBI) 55432
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ5VVQ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YOD1, also known as YOD1 deubiquitinase, belongs to the ovarian tumor (OTU) domain-containing family of deubiquitinating enzymes (DUBs), which play a crucial role in regulating cellular processes by removing ubiquitin molecules from target proteins. YOD1 is particularly involved in the endoplasmic reticulum-associated degradation (ERAD) pathway, where it assists in the clearance of misfolded proteins from the endoplasmic reticulum (ER) by modulating the ubiquitination status of ERAD components. (PMID: 40132762,19818707)

实验方案

Product Specific Protocols
IF protocol for YOD1 antibody 84933-5-RRDownload protocol
WB protocol for YOD1 antibody 84933-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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