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验证数据展示

  • WB analysis using 84851-4-RR (same clone as 84851-4-PBS)

    WB analysis using 84851-4-RR (same clone as 84851-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84851-4-RR (Complement factor D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

    IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84851-4-RR (Complement factor D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

    IHC staining of mouse stomach using 84851-4-RR (same clone as 84851-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84851-4-RR (Complement factor D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01660-3

    Sandwich ELISA standard curve of MP01660-3

    Sandwich ELISA standard curve of MP01660-3, Human Complement factor D Recombinant Matched Antibody Pair - PBS only. 84851-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1189. 84851-4-PBS was HRP conjugated as the detection antibody. Range: 4.69-300 pg/mL.

  • Sample test of MP01660-3

    Sample test of MP01660-3

    Serum of eight individual healthy human donors was measured. The Complement factor D concentration of detected samples was determined to be 838.5 ng/mL with a range of 561.9-1525.4 ng/mL.

  • IF Staining of THP-1 using 84851-4-RR (same clone as 84851-4-PBS)

    IF Staining of THP-1 using 84851-4-RR (same clone as 84851-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using Complement factor D antibody (84851-4-RR, Clone: 242244C2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84851-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84851-4-RR

    Affinity and Kinetic Characterization of 84851-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84851-4-RR against Human Complement factor D were performed. The affinity constant is 60.2 pM.

Complement factor D Recombinant monoclonal antibody, PBS Only (Detector)

Complement factor D Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84851-4-PBS

产品说明书

CloneNo. 242244C2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

别名

CFD, 242244C2, ADIPSIN, C3 convertase activator, complement factor D (adipsin)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84851-4-PBS targets Complement factor D as part of a matched antibody pair:

MP01660-3: 84851-2-PBS capture and 84851-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg1189

Product name: Recombinant Human Complement factor D protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 26-253 aa of NM_001928.3

Sequence: ILGGREAEAHARPYMASVQLNGAHLCGGVLVAEQWVLSAAHCLEDAADGKVQVLLGAHSLSQPEPSKRLYDVLRAVPHPDSQPDTIDHDLLLLQLSEKATLGPAVRPLPWQRVDRDVAPGTLCDVAGWGIVNHAGRRPDSLQHVLLPVLDRATCNRRTHHDGAITERLMCAESNRRDSCKGDSGGPLVCGGVLEGVVTSGSRVCGNRKKPGIYTRVASYAAWIDSVLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 complement factor D (adipsin)
别名 CFD, 242244C2, ADIPSIN, C3 convertase activator, complement factor D (adipsin)
计算分子量27kDa
观测分子量 27 kDa
GenBank蛋白编号NM_001928.3
基因名称 CFD
Gene ID (NCBI) 1675
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP00746
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Complement factor D, also known as adipsin, is a highly specific serine protease. Complement factor D is the rate-limiting enzyme in the alternative pathway of complement. It activates C3 convertase by cleaving factor B, which is a key step in the alternative pathway. Complement factor D catalyzes the cleavage of factor B to generate Ba (non-catalytic chain) and Bb (active catalytic subunit), which together with C3(H2O) (or C3b) constitute the active C3 convertase C3(H2O)Bb (or C3bBb). (PMID: 34566967; PMID: 27775713).

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